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Related posts:. Yield now.Such vectors allow for self-replication, inside the cells, and potentially integration in the host DNA. This will be used to prime the synthesis of a complementary DNA strand by reverse transcriptase. Isolation of chick embryo total RNA 1. The mRNA is used to make viral proteins to take over the host cell. Combining oligo dT and random primers is effective in overcoming the disadvantages associated with each mechanism of priming. It is likely that the ssDNA forms a hairpin loop at the 3' end.
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